Prokaryotic expression with PTDeGFP fusion proteins of different molecular weight transmembrane Ability.doc

 PAGE \* MERGEFORMAT 13 Prokaryotic expression with PTDeGFP fusion proteins of different molecular weight transmembrane Ability Of: Tian Xin Chen, Hong Xu Xun Xun Lin Yang Yong move Qianqian quarter were Po River Shaoqi Xiang Ju Wang Shengjun promise of [Abstract] Objective: To evaluate the HIV 1 TAT protein transduction domain (PTD) of different molecular weight of carrying the fusion protein through the cell membrane capacity. Methods: Induction of expression and purification of PTD eGFP containing the different molecular weight of the fusion protein, and cell co-culture later, by flow cytometry, confocal evaluation of penetrating ability of the fusion protein. Results: The induction of expression, purified with 3 different molecular weight PTD eGFP fusion protein. flow cytometry and confocal analysis found that 3 fusion proteins all have high penetrating ability. Conclusions: PTD-mediated membrane fusion proteins ability to wear no direct relation with the molecular mass. [Keywords:] protein transduction domain, transmembrane efficiency, enhanced green fluorescent protein, molecular weight [Abstract] Objective: To judge the efficacy of the fusion protein including protein transduction domain of HIV TAT by multi biotechnology and to obtain the more effective cellular uptake protein for the further research of its bio function.Methods: The expression of three fusion proteins were induced by IPTG and were purified from the inclusion bodies. Flow cytometry analysis and confocal microscope observation were used to evaluate the efficacy of the fusion proteins to transduce into the Jurkat cells, when the cells were exposed to fusion protein. Results: The fusion proteins could transduce into Jurkat T cells efficiently. Conclusion: All the three fusion proteins could cross the cell membrane and had no difference. [Keywords:] protein transduction domain; the efficacy of trans membrane; prokaryotic expression, green fluorescence protein 1988, Green an