Prostatic intraepithelial neoplasia in the microvessel density DNA ploidy immunophenotype and their biological characteristics.doc

 PAGE \* MERGEFORMAT 14 Prostatic intraepithelial neoplasia in the microvessel density DNA ploidy immunophenotype and their biological characteristics Of: Houhui Lian, Zhang Xuebin, Li Xu, ZHANG Guan-jun, Hong-Yan Wang, Deng Yuan, Wang [Abstract] Objective To investigate microvessel density (MVD), DNA ploidy, immunophenotype, and prostatic intraepithelial neoplasia (PIN) and its biological characteristics. Immunohistochemical SP method and computer image analysis method, 24 cases of benign prostatic hyperplasia (BPH), 23 cases of low grade prostatic intraepithelial neoplasia (LGPIN), 26 patients with prostate cancer (PC) associated with high-grade prostatic intraepithelial neoplasia (HGPIN) in paraffin-embedded specimens CD34, 34E12, P63, P504s expressed detection, quantitative determination of the MVD and nuclear DNA ploidy. the result of different levels of PIN in the MVD, and DNA ploidy were significantly higher than the average BPH group, but significantly lower than the PC group (P lt;0.01), and with the PIN level increased, Different DNA ploidy in polyploid values, with an increasing number and MVD (P lt;0.01). DNA ploidy and positive correlation between MVD and P63 .34 El2 glands in BPH and LGPIN continuous around the basal cells were positive in HGPIN was intermittent in the positive, was negative in the PC. P504s was spotty in the HGPIN positive, strongly positive in the PC, in BPH and LGPIN in the negative. Conclusion DNA ploidy, MVD is closely related with the PIN is a reflection of its important reference for the biological characteristics, immunophenotype, especially further confirmed HGPIN is a PC PIN the precancerous lesions. [Keywords:] prostatic intraepithelial neoplasia, prostate cancer, microvessel density, DNA ploidy, immunohistochemistry ABSTRACT: Objective To study microvascular density (MVD), DNA ploidy and immunophenotype in prostatic intraepithelial neoplasia (PIN) as well as their relationship with its biological properties