Pueraria flavonoid extract of HL 60 cell proliferation and apoptosis.doc

 PAGE \* MERGEFORMAT 11 Pueraria flavonoid extract of HL 60 cell proliferation and apoptosis Authors: Huai-Bo, Man-Tian Mi, Chen Chung Road, Wei Zhaojun Keywords: cell proliferation College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; Department of Nutrition and Food Hygene, 3rd Military and Medical University; College of Food Science, Southwest University Abstract: Objective To investigate the effect of Kudzu flavonoids extracts on human leukaemia cancer cells HL  60 line to reveal its probable mechanism. MethodsThe survival and proliferation of HL  60 cells were determined by MTT.The cell  DNA ploidy distribution and apoptotic rate were measured by flow cytometry (FCM), and DNA ladder.The differentiation induced potence was determined by NBT. The anticancer and apoptosis potence induced by flavonoids detected by TUNEL. ResultsThere was significant inhibition of the cel1 surviva1 and proliferation of cancer cells by the extracts. An apoptosis peak appeared after treated with 3d and apoptotic rate was 38.3%, morphological change and DNA ladder of apoptosis was observed. Kudzu flavonids showed weak potent to induce differentiation. Daidzein and puerarin which regard as Kudzu flavonoids main effect ingredient have weak apoptosis induced potent , but daizein showed strong differentiation induced potent. ConclusionKudzu flavonoids could significantly inhibit growth of human leukaemia cancer cells HL  60 line through inducing apoptosis. Keywords:: Kudzu; Flavonoids extracts; Apoptosis; Differentiation Abstract: Objective To observe the flavonoid extract of Pueraria lobata HL  60 cells and its mechanism. Methods MTT determination in HL  60 cell proliferation; using flow cytometry cell cycle changes and apoptosis; using NBT colorimetric detection of HL  60 cells induced by the ability to; DNA Ladder Detection of HL  60 cells apoptotic features and effects; in vivo induction