Pure glycerin -4 ℃ amniotic membrane activity of preservation methods on the study.doc

 PAGE \* MERGEFORMAT 2 Pure glycerin -4 ℃ amniotic membrane activity of preservation methods on the study Author: Zhang Jun-Rong Cui Wei Gao Wei, animal husbandry, [Abstract] Objective: To observe -4 ℃ glycerol preservation method of the amniotic membrane activity. Methods: Healthy cesarean section maternal placenta, amnion stripped, divided into 2cm * 2cm pieces and stored at -4 ℃ pure glycerine, 3,7,14,30,60,90 d later, respectively, by light microscopy, enzyme histochemistry [lactate dehydrogenase (LDH), succinate dehydrogenase (SDH)] and immunohistochemical [basic fibroblast growth factor (bFGF)] observations, and in contrast to the fresh amniotic membrane. Results: The light microscope, -4 ℃ glycerol preserved amniotic membrane epithelium 60d within the structure of a more complete, we can see a small number of cell rupture. Nucleus Tuoshi. 90d group structural damage evident on the cortex, most cells rupture, nuclear Tuoshi. Enzyme histochemistry (LDH, SDH) and immunohistochemistry (bFGF) Show: 60d within the group of fresh amniotic membrane was no significant difference; 22 Comparison between the groups there were no significant differences (P