Qi Chinese Herbal Medicine on diabetic skin ulcers in rats the effects of transforming growth factor-β 1.doc

 PAGE \* MERGEFORMAT 15 Qi Chinese Herbal Medicine on diabetic skin ulcers in rats the effects of transforming growth factor-β 1 Authors: Zhen Zhang, Quehua hair, Zhuyuan Ying, Yun-Fei Wang, Xiao-Dong Liu, Pei-Yong Zheng [Keywords:] Diabetes mellitus; skin ulcers; transforming growth factor β 1; rat Diabetic ulcers are a common complication of diabetes. Qi of Chinese Herbal Medicine treatment of diabetic ulcers has achieved good efficacy [1], in order to further explore the promotion of wound healing in diabetic ulcers mechanism, carried out the study. 1 Materials and methods 1.1 Experimental Animal 2-month-old SD rats of clean grade 60, male, body mass 200 ~ 250 g, average (223.05 ± 14.91) g, from the Shanghai Experimental Animal Center of Chinese Academy of Sciences. Animal Qualified Certificate No.: medical action word first 02  22  2 Hao. 1.2 Drug Yiqi Huayu Recipe by the Health and Astragalus, heterophylla, peach kernel, and to the dragon, the dose ratio of 2.5:2.5:1:1 rats at a dose of 11.61 g / (kg * d), vacuum concentrated to the concentration of containing crude drugs for the 2.5 g / ml. Fang Qi Fang demolished by the Health and composed of Radix Astragali and Taizishen rats at a dose of 6.75 g / (kg * d), vacuum concentrated to a concentration of crude drugs containing 1.5 g / ml. Huayu side by side split peach kernel and real dragon, rat in a dose of 4.86 g / (kg * d), vacuum concentrated to a concentration of crude drugs containing 1 g / ml [2]. By the Longhua Hospital, Shanghai University of Traditional Chinese Medicine preparation room by decoction decoction was prepared by alcohol precipitation process enrichment, 4 ℃ refrigerator spare. Basic fibroblast growth factor (basic fibroblast growth factor, bFGF) bFGF, Zhuhai East largest bio-pharmaceutical Co., Ltd. products (approval number: National medicine accurate . 1.3 Main experimental reagents transforming growth factor-β 1 (transforming growth factor  β 1, TGF  β