Qi and yin Chinese Xiaozheng Tongluo rat mesangial cell secretion of extracellular matrix in.doc

 PAGE \* MERGEFORMAT 11 Qi and yin Chinese Xiaozheng Tongluo rat mesangial cell secretion of extracellular matrix in Of: Jiang-Hua Zhang 1, Zhao Wen Hung Chen Zhiqiang Sun Yufeng Li Yumei Yue-Hua Wang Wang Huiqing [Abstract] Objective Yiqiyangyin, Xiaozheng meridians of Chinese medicine serum on rat mesangial cell extracellular matrix (ECM) inhibitory effect of different components. Methods of in vitro cultured rat mesangial cells and serum pharmacological Methodology is based on an enzyme-linked immunosorbent assay ECM fibronectin (FN), laminin (LN), collagen (Col IV) contents. Results FN, Col levels were increased, while Qi and yin, xiao Tongluo Herbs have significant inhibitory effect. Conclusion Yiqiyangyin, Xiaozheng Tongluo drugs to reduce the accumulation of ECM, may be the mechanism of prevention of glomerular sclerosis. [Keywords:] mesangial cells; Qi Yin, Xiaozheng Tongluo; laminin; fibronectin; collagen; extracellular matrix [Abstract] Objective To investigate the inhibitory effect of herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals on the mesangial cells (MCs) secreting extracellular matrix (ECM). Methods Based on the method of culturing MCs in vitro and serum pharmacology, the contents of fibronectin (FN), laminin (LN) and collagen (Col ) in ECM were detected by ELISA.Results The contents of FN, LN, Col in ECM were all increased, while inhibited by herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals significantly. Conclusions Herb of supplement qi and nourish yin eliminating mass and dredge nels and collaterals can decrease the accumulation of ECM, which may be one of the mechanisms of preventing glomerulosclerosis. [Keywords:] Mesangial cells; Supplement qi and nourish yin eliminating mass and dredge nels and collaterals; Laminin; Fibronectin; Collagen ; Extracellular matrix Diabetic nephropathy (DN) of basic pathological changes of the ball as early gl