Qingdu suppository in cervical cancer SiHa cells and PTEN-MDM2-p53 loop of the network.doc

 PAGE \* MERGEFORMAT 13 Qingdu suppository in cervical cancer SiHa cells and PTEN-MDM2-p53 loop of the network [Abstract] Objective cell biology and molecular biology from the level of disinfection tied to cervical cancer SiHa cell proliferation and the PTEN-MDM2-p53 protein expression in each network. Methods of 4% serum containing medium effect on SiHa cells, normal control group, blank serum, Qingdu bolt group Baofukang suppository group and the interferon group, by MTT assay containing serum on inhibition of cell proliferation, while the use of Western blot, PTEN-MDM2 -p53 protein expression. Results The effect of serum containing medium reduced the number of cells in each group after which the concentration of 4% serum containing 72 h, the strongest inhibition when cells with blank serum group, the difference was statistically significance (P lt;0.01), while containing serum increased PTEN, P53 tumor suppressor protein, reducing the expression of oncoprotein MDM2. Conclusion Qingdu suppository containing serum may be through regulation of PTEN-MDM2-P53 Network Ring Road and inhibit tumor purpose. [Keywords:] Qingdu Suppository, cervical cancer, SiHa cells, PTEN-MDM2-p53 network Abstract: Objective To discuss the effect of Qingdushuan on SiHa cells of cervical cancer proliferation and protein expression in PTEN-MDM2-p53 network from the level of cell biology and molecular biology. Methods SiHa cells were cultivated with 4% containing serum. Normal control group , blank serum group, Qingdushuan group, Baofukang group and interferon group were established. The influence of inhibiting and proliferating SiHa cell was detected by MTT assay. Three protein expression was detected by Western blot method. Results After cultured with drug containing serum, the number of cells decreased, 4% concentration of serum for 72 h was the strongest. Compared with the control group, there were significant differences (P lt;0.01). At the same time, drug containing serum