Rational screening high-yielding strains of penicillin.docVIP

 PAGE \* MERGEFORMAT 11 Rational screening high-yielding strains of penicillin Author: Lou Xin Zhang Li Chen Lihua Liu Jing Liu Jing ZHANG Yu-lian He Jiangong [Abstract] order penicillin production strains 87117 # as the starting strain, using UV mutagenesis precursor complex dynamic post-processing methods, combined with the limiting oxygen flask under the conditions of screening, 177 for supporting the front from the body of resistant strains of weed out the XY -137 # high-yield mutant. The strain shake-flask titers an average of 8.8%, and the genetic characteristics of a stable, unit mycelium product synthesis ability. XY-137 # strain in the tank through the nearly 100 tons of production batch verification, its place pot potency, fermentation index, tank batch production increased by an average 3.83%, 5.66% and 2.69%. [Keywords:] UV mutagenesis; dynamic post-processing; rational screening; high-yielding strains of penicillin ABSTRACT Using Penicillium chrysogenum # 87117 as starting strain, a penicillin high yield strain # XY-137 was obtained by UV irradiation combined with precursor-resistant strain in shake flask selection method under limited oxygen. Penicillin potency of strain # XY-137 was increased by 8.8% cultured in shaking flasks. Average productivity of about 100 batches fermentation level was increased by 2.69% ~ 5.66% than that of strain # 87117 in 100-ton fermentor. KEY WORDS UV mutagenesis; Dynamic post-treatment; Rational screening; Penicillin high-producing strain Because penicillin post-processing of raw materials and semi-synthetic field increasingly wide range of penicillin in the world market is fiercely competitive. Producers only to continually enhance the level of production, lower production costs in order to enhance the competitiveness of enterprises, while the high-quality and application of high-yielding strain and is one of the foundation and the key. With the production capacity of penicillin strain improvement of the limi