Quercetin inhibited cell proliferation of gastric cancer BGC823.docVIP

 PAGE \* MERGEFORMAT 10 Quercetin inhibited cell proliferation of gastric cancer BGC823 Author: Xu Fan to Yu, YAO Guang-Tao, Li Yi-Kui [Abstract] Objective To investigate the quercetin treatment of gastric cancer cells in rats. Methods with different concentrations of quercetin BGC823 treatment of gastric cancer cells using MTT colorimetric test for detection cancer cell growth, respectively, by agarose gel electrophoresis and TUNEL detection of apoptosis, respectively, by fluorescent real-time quantitative PCR and Western blot Detection of survivin (survivin) gene mRNA, protein expression. Results quercetin inhibited the proliferation of malignant cells in gastric cancer BGC823, and a dose-dependent manner; agarose gel electrophoresis and TUNEL showed that apoptosis in quercetin-treated group phenomenon, and a dose-dependent. Quercetin treatment group survivin gene mRNA, protein expression down, and a time-and concentration-dependent. Conclusion Quercetin can inhibit gastric cancer cells in vitro malignant proliferation, and induction of apoptosis, down survivin gene expression. [Keywords:] gastric cancer cell apoptosis survivin quercetin Abstract: ObjectiveTo identify the inhibitory effects and mechanism of Quercetin on gastric cancer.MethodsGastric cancer BGC823 cells were treated with different doses of quercetin, and the growth of cancer cell was exmined by MTT assay, and the apoptosis were determined by agarose gel electrophoresis and TUNEL assays, respectively.The real time-PCR and western blot were performed to study the expression of survivin.ResultsQuercetin had a remarkable inhibition on gastric cancer cell line BGC823.From DNA ladder and TUNEL assay, apoptosis were induced in cancer cells treaed with quercetin, in a dose-dependent manner.In addition, quercetin resulted in down-regulation of survivin mRNA and protein from real time-PCR and western blot assay.ConclusionQuercetin can inhibit the growth of human gastric cancer BGC823 cell through in