RCC OSRC2 expression and significance of CD133 cells.docVIP

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RCC OSRC2 expression and significance of CD133 cells.doc

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RCC OSRC2 expression and significance of CD133 cells

RCC OSRC2 expression and significance of CD133 cells Of: from the Sun Hao Tian Fu, Pan Peng, Guo Tao [Abstract] Objective: Stem Cells cultured renal cell carcinoma, the molecular characteristics of the surface. Methods: The renal cancer cell suspension containing epidermal growth factor (epidermal growth factor, EGF) and fibroblast growth factor (basic fibroblast growth factor, bFGF)-free cultured in serum medium, cells were collected and cultured for 7 days after the ball, flow cytometry the expression of CD133 and CD34. Results: Suspension culture renal stem cells appeared 2 days after the ball, the ball stem cells cultured for 7 days significantly increased, flow instrument detected the expression of CD133 + CD34-cells approximately (8.33 + -1.26)% and control the expression of renal cell carcinoma group only CD133 + CD34-cells (1.24 + -0.36)%; Stem Cells containing 10% fetal bovine serum by train base continues to develop, and restore the original growth characteristics. Conclusion: The serum-free suspension culture of kidney stem cells obtained by the ball surface expression of stem cell marker CD133. [Keywords:] cancer stem cells; renal cell carcinoma; cell culture; CD133; CD34 [Abstract] Objective: To cultivate renal cell carcinoma (RCC) stem cells in serum free medium (SFM) with the method of suspension culture and to research their property of surface molecules on the cells.Methods: RCC cells were collected and dissociated in to single ones, then they were seeded in SFM containing EGF and bFGF. Seven days later the expression of CD133 and CD34 in cells from SFM was detected by flow cytometry. Results: RCC stem cells spheres were appeared after RCC cells had been cultured two days in SFM; Seven days later, more spheres came out. Then collected and detected them by flow cytometry. We found that about (1.24 + -0.36)% CD133 + CD34-cells were in normal OS RC 2 cell line, while it reached to ( 8.33 + -1.26)% after cultivating in SFM for 7 days. After