Recombinant human ciliary neurotrophic factor (rhCNTF) short-term application of sodium glutamate obese rats on the effects of lipid.doc
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Recombinant human ciliary neurotrophic factor (rhCNTF) short-term application of sodium glutamate obese rats on the effects of lipid
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Recombinant human ciliary neurotrophic factor (rhCNTF) short-term application of sodium glutamate obese rats on the effects of lipid
Author: Shu-Fang Guo, Han Guozhu, Li Weiping, Zhou Qin
[Abstract] Objective To study the short-term application of sodium glutamate obese rats rhCNTF lipid effects. Methods MSG rats: Birth d1 of the SD rats of either sex, subcutaneous injection of sodium glutamate (MSG) 3mg / (g * d), for 5 days, the normal saline control group, subcutaneous injection of equal volume. 3-month-old will be obese animals were randomly divided into five groups: subcutaneous injection rhCNTF300μ g / (kg * d) (the high-dose group), 100μ g / (kg * d) (medium-dose group), 30μ g / (kg * d) (low dose group), sibutramine gavage 8mg / (kg * d) (positive control group), dilute solution 1.0ml / (kg * d) (obese control group); model group and the control group were given normal saline 1ml / (kg * d), consecutive administration for 10 days. After the last administration 24h, while animals after overnight fasting, in the chloral hydrate anesthesia, the measured body weight, body length, calculate LI; decapitated after the taking of abdominal fat weighed to calculate fat index (fat weight / body weight); abdominal aorta blood serum obtained after centrifugation was measured total cholesterol (cholesterol oxidase - peroxidase), triglycerides (GPO-PAP method), high-density lipoprotein (sedimentation method), glucose (GOD-PAP method) and serum insulin (radioimmunoassay) levels; decapitated after the taking of abdominal fat weighed to calculate fat index (fat weight / body weight). Results ⑴ modeling: normal rats (189.40 ± 38.72) g, average weight of sodium glutamate obese rats (246.72 ± 36.67) g, (P
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