Rapid detection of organophosphorus pesticide residues Research Progress.doc

 PAGE \* MERGEFORMAT 5 Rapid detection of organophosphorus pesticide residues Research Progress [Keywords:] Organic phosphorus pesticides Pesticide residue testing technology was originally limited to chemical methods, colorimetry, and biological assay, detection methods lack of specificity, sensitivity is not high. The 20th century, 60 years gas chromatography used in pesticides and drug residue analysis, greatly increased the amount of pesticides and drug residue detection level. Since the 20th century, 80 years, high performance liquid chromatography have been widely applied to the thermal instability and ion-type pesticide and its metabolites analysis. Although the precise quantitative chromatography, high sensitivity, but the necessary equipment expensive, require professional operation, and the analysis of a long time is not conducive to on-site monitoring. In this paper, the current rapid detection of pesticides and drug residue analysis technology research progress of a review. A light-emitting bacteria detection technology Studies have shown that different types of light-emitting bacteria in the same light-emitting mechanism 〔 1〕 . That is, from the role of molecular oxygen, intracellular fluorescent enzyme catalysis, will restore the state of the flavin nucleotide (FMNH2) and long-chain fatty aldehyde is oxidized to FMN and long-chain fatty acids, while the release of the greatest luminous intensity at a wavelength of 450 ~ 490nm blue-greenLight . Commonly used light-emitting bacteria are Vibrio and Photobacterium some of the bacteria. Yuan Dongxing 〔 2〕 , who uses light-emitting bacteria rapid detection of organophosphorus pesticides in vegetables residues in vegetables by light-emitting bacteria on the suppression of several organophosphorus pesticides light response obtained luminous intensity and the concentration of organophosphorus pesticides in samples negative relevant results, the minimum detection limit up to 3mg / L. At present, the lig