Quality standard of thistle_0.doc

 PAGE \* MERGEFORMAT 10 Quality standard of thistle [Abstract] Objective To establish quality standards for medicines thistle. Methods TLC of Micro linarin qualitative identification; by high performance liquid chromatography linarin content column for the Ultimate Column XB-C18 (4.6 mm × 250 mm, 5 μm), mobile phase was methanol - water (51:49), detection wavelength was 334 nm, flow rate 1.0 ml · min-1, the column temperature was 25 ℃. the results of TLC spots were clear and repeated good; high performance liquid chromatography linarin 0.006 58 ~ 2.964 μg in peak area between the injection volume was linear (r = 0.999 9), the average recovery was 100.7%, RSD 1.89 %. Conclusion The method is simple and feasible, reproducible, and can be used as quality control of medicinal thistle. [Keywords:] thistle; linarin; quality standards; TLC; high performance liquid chromatography Abstract: ObjectiveTo establish the quality standard of Herba Cirsii. MethodsA TLC method was used for qualitative identification and at HPLC analysis was applied for quantitative determination of Herba Cirsii with linarin as the reference substances. The column was Ultimate Column XB-C18 (4.6 mm × 250 mm, 5 μm), the mobile phase was the mixture of methanol-water (51:49) with a flow rate of 1.0 ml · min-1, the UV detection wavelength was 334 nm and the column temperature was set at 25 ℃ . ResultsThe TLC method was suitable for the identification of Herba Cirsii. A good linear relationship between the peak areas and injected amounts of linarin in the range of 0.006 58-2.964 μg, the average recovery was 100.7% (RSD = 1.89%). ConclusionThe established method is simple and reproducible and can be used as quality control methods of Herba Cirsii. Keywords:: Herba Cirsii; Linarin; Quality standard; TLC; HPLC Thistle thistle Asteraceae plants as Cephalanoplos Cirsium setosum (Willd.) MB.’s Dried aerial parts, are distributed all over the country, with a cooling blood to stop bleeding, b