Qi decoction on gastric precancerous lesions of rat protein kinase C and cyclin E protein expression.doc
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Qi decoction on gastric precancerous lesions of rat protein kinase C and cyclin E protein expression
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Qi decoction on gastric precancerous lesions of rat protein kinase C and cyclin E protein expression
[Abstract]: Objective Yiqi Huayu Recipe on rats with gastric precancerous lesions of gastric mucosa of protein kinase C (PKC), cyclin E (CyclinE) rats. Methods A nitro-nitro-guanidine (MNNG), ethanol, irregular diet and other factors making model. After the modeling, the surviving rats were randomly divided into the modeling of natural recovery group and Chinese medicine with high, medium and low dose group, the rats treated accordingly, immunohistochemistry used to detect the gastric mucosa of rats PKC, CyclinE expression. Results of the treatment group CyclinE PKC protein expression and a more natural recovery group the positive rate of decline, were significantly different (P lt;0.05). Conclusion Qi decoction may be by decreasing the expression of PKC and CylinE, inhibit cell proliferation, induce apoptosis, and play in rats the treatment of gastric precancerous lesions.
[Keywords:] protein kinase C precancerous lesions of cyclin E in rats Qi decoction
Abstract: Objective To observe the effect of Yiqi Huayu Recipe on the expression of protein kinase C (PKC) and CyclinE in rats with gastric precancerous lesions (GPL). Methods The model of GPL was established mainly through N-methyl-N-nitro N -nitrosoguanidine (MNNG) together with other factors including alcohol stimulation and undue hunger and overeating. The survived rats after modeling were randomly divided into spontaneous recovery, and high-, moderate-and low-dose Yiqi Huayu group. After the corresponding treatment, the expression of PKC and CyclinE were detected by immunohistochemical method in each group. Results The expression of PKC and CyclinE in trement group were distinctly lower than those in spontaneous recovery group (P lt;0.05). Conclusion Yiqi Huayu Recipe was effective in the treatment of rats with GPL by inhibiting the expression of PKC and CyclinE.
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