Recombinant human growth hormone on the growth of gastric cancer xenografts in nude mice and serum levels of VEGF.docVIP

 PAGE \* MERGEFORMAT 19 Recombinant human growth hormone on the growth of gastric cancer xenografts in nude mice and serum levels of VEGF [Abstract] Objective: To study the recombinant human growth hormone (rhGH) on growth of human gastric tumor growth and serum VEGF levels. Methods: 60 male nude mice experiment, by immunocytochemical staining of gastric cancer cell line SGC 7901 and MKN 45 growth hormone receptor (GHR) expression status, the expression of GHR-positive gastric cancer cell line SGC 7901 and GHR negative gastric carcinoma cell line MKN 45 were inoculated in four nude mice, producing gastric cancer transplanted subcutaneously in nude mice tumor model, 3 weeks after the tumor made of tissue removed, the remaining 52 were inoculated into nude mice. according to vaccination into tumor cells of different types of GHR expression and negative expression of GHR in nude mice, nude mice with different expression of the further sub-GHR the control group (C group: to 0.9% NaCl, 0.2 ml d-1), rhGH low dose group (L group: I rhGH 0.5 U kg-1 d-1), rhGH high dose group (H group: to rhGH 2.5 U kg-1 d-1), continuous administration of 14 d, mice in each group were observed and recorded body weight and tumor volume changes, enzyme-linked immunosorbent assay of serum VEGF levels. Results: SGC 7901 is positive for GHR expression in human gastric cancer cell lines, MKN 45 negative for the expression of GHR in human gastric cancer cell lines. GHR expression for the inoculation of human gastric cancer cell lines in nude mice, rhGH administration of subcutaneous tumor size larger than the control group (P lt;0.05 ), and promote the growth effects of H group was significantly (P lt;0.05), 3 weight was no significant difference between groups (Pgt; 0.05), serum VEGF detection, L group (167.60 + -9.54 pg ml-1, H group ( 252.94 + -15.32 pg ml-1, compared with C group (49.94 + -5.73 pg ml-1 increased (P lt;0.05), negative for the expression of GHR inoculation of huma