Recombinant parvovirus on glioma cell growth inhibition.docVIP

 PAGE \* MERGEFORMAT 18 Recombinant parvovirus on glioma cell growth inhibition Abstract [Objective] To study the expression of chemokine MIP-1α/LD78β recombinant parvovirus on glioma cell growth inhibition. [Methods] prepared by co-transfection recombinant virus. With the recombinant virus infection of human glioma cell lines U87MG and rat glioma cell line GL261, using ELISA to monitor cell growth and cell culture supernatants were measured MIP-1α protein expression in the daily and cumulative. Experimental Determination of colony-forming virus cytotoxicity. [Results] Compared with untreated cells, MOI = 3 RU / cell of recombinant virus infection to reduce the formation of GL261 cell clones. U87MG, and GL261 cells infected with recombinant virus could produce a large number of MIP-1α protein. Day peak of expression after infection were in 3d and 4d. 2 × 105 of the GL261, and U87MG cells infected (MOI = 3) 5d after the cumulative amount of protein expression were 780ng/ml and 250ng/ml. Compared with the control, recombinant virus-infected GL261 cell growth slowed down, but the expression of MIP-1α or LD78β of the recombinant virus with and without exogenous gene after recombinant virus infection of GL261, no significant difference in the number of living cells. U87MG the sensitivity of the virus below the GL261, infected with recombinant viruses of each group slightly slower than that of control cell growth. [Conclusion] The recombinant parvovirus U87MG and GL261 in vitro inhibit the growth of glioma cells, but MIP-1α/LD78β exogenous gene transduction in vitro had no significant effect on cell growth, inhibition was mainly due to the virus-cell cause toxicity. Keywords: recombinant parvovirus cytotoxic growth inhibition of glioma cell Growth Inhibition of Glioma Cells after Infection with the Recombinant Pavoviruses Malignant glioblastoma is the most common primary central nervous system tumors, conventional treatment falls far short