Recombinant thymosin 4 expression of NFB regulation to promote repair after corneal alkali burn.docVIP

 PAGE \* MERGEFORMAT 17 Recombinant thymosin 4 expression of NFB regulation to promote repair after corneal alkali burn Study: Lung Wah, Li Ming, Wang Xiaokun, Mr Man, Li Xuande, Ya Nie, Masu Yong, car Yong Zhe Xu Matsuyama [Abstract] Objective: To study the recombinant thymosin 4 (Thymosin 4, T4 corneal alkali burn of cornea nuclear transcription factor B (nuclear factor B, NF B expression and the promotion of corneal healing observed. Methods: 1mol / L NaOH saturated The 6mm diameter circular filter paper affixed to the rabbit corneal 30s, rabbit corneal alkali burn model. animals were randomly divided into experimental and control group, low in the experimental group received high dose (0.1,1,10 g/50LT4, the control group received PBS ( negative control group and rh EGF (positive control group were 2 times / d drops. burn 1, 3, 7, 14d naked eye changes in corneal burns, photographic, statistical healing rate, immunohistochemical detection of NF B, IL 1 expression, burn areas to take liquid extraction of corneal tissue nuclear protein detected by ELISA in the p65 subunit of NF B expression. Results: The treatment of corneal alkali burns T4 the highest rate of corneal healing in 33.8%, the corresponding negative control group was 22.8% , T4 treatment group was significantly higher (P lt;0.01, one dose of T4 group healing rate in the other dose groups, T4 drug group NF B immunohistochemistry integral optical density was significantly lower than the control group (P lt;0.05, ELISA Detection of the p65 subunit of NF B in the expression of at least T4 drug group (P lt;0.01, IL 1 immunohistochemical staining, but the experimental group and control group was not statistically significant. Conclusion: The recombinant T4 promote corneal alkali burn repair, The mechanism may be related to decreased NF B in the nucleus of the expression. [Keywords:] alkali burn; wound healing; thymosin 4; matrix metalloproteinase-2; vascular endothelial growth factor Abstr