Rat bone marrow derived liver stem cells to settle status of liver transplantation.docVIP

 PAGE \* MERGEFORMAT 10 Rat bone marrow derived liver stem cells to settle status of liver transplantation Of: Science Zhao Ji Wang Guangyi Zhang Haiyu [Abstract] Objective To explore the bone marrow derived liver stem cells after allograft rat model of liver receptor for settlement. Methods using whole bone marrow cells cultured in vitro adherence screening purified mesenchymal stem cells in bone marrow and in vitro cultured and amplified, the application of hepatocyte growth factor (HGF) induced differentiation of liver stem cells, identified by the liver allograft was injected into the middle of the normal group and fulminant hepatic failure in rats in vivo, after transplantation, 1,2 4 weeks to take receptor in situ liver transplantation (injection site), o-(from the injection site 0.5 cm) organization, application of PCR to detect sex-determining gene fragment of liver stem cells to settle in the liver. Results after liver injection of liver stem cell transplantation and liver injury in the normal group could settle in the liver, and the number of cells settled on the liver injury was significantly more than the normal group. Conclusions Transplantation of bone marrow-derived liver injection of liver stem cells can settle in the liver , and set the number of stem cells in the liver and liver injury is closely related. [Keywords:] bone marrow mesenchymal stem cells, liver stem cells, cell transplantation Mesenchymal stem cells (MSCs) have multilineage differentiation potential, can induce in vitro differentiation of hepatic stem cells, such bone marrow-derived liver stem cell transplantation for liver tissue engineering offer new sources of cells. In recent years research has focused on the in vitro MSCs induced liver stem cells, such cells have the morphology of liver cells in vitro can express some liver cell function (1), but bone marrow-derived liver stem cells in the body of the settlement and the situation is not clear positioning capabili