Release of exogenous CO on pulmonary hypertension molecular NO synthase activity of rat.doc

 PAGE \* MERGEFORMAT 17 Release of exogenous CO on pulmonary hypertension molecular NO synthase activity of rat Of: Zhang Yuan Jufang win for Shao Donghua Sun Bingwei [Abstract] Objective: To observe the release of exogenous CO molecule (CORM 2) on monocrotaline (monocrotaline, MCT)-induced pulmonary hypertension (pulmonary artery hypertension, PAH) and its rat NO synthase (NOS). Methods : Adult male SD rats, single intraperitoneal injection of MCT 60 mg / kg. the first 15 days measured by the internal jugular vein catheterization right ventricular systolic pressure (sRVP), successfully screened rat model of pulmonary hypertension 40. this Big 40 mice were randomly divided into 4 groups: model group, high dose group, low dose group, no active group, Another 10 normal rats as normal control group, a total of 5 groups. The first 21 days of taking the test lung tissue NO , eNOS and iNOS. Results: Compared with the control group, model group, non-active group of NO decreased significantly (P lt;0.05); with the model group, high dose group, low dose group, NO increased significantly (P lt;0.01) . Compared with the control group, model group, non-active group, low dose group was significantly decreased eNOS, iNOS was significantly higher (P lt;0.05); with the model group, high dose group, low dose group increased eNOS, iNOS decreased (P lt;0.01). CONCLUSION: CORM 2 treatment of PAH, possibly by regulating eNOS, iNOS activity and thus affect the production and release of NO to finish. [Keywords:] CO releasing molecules; pulmonary hypertension; nitrous oxide; nitric oxide synthase [Abstract] Objective: To investigate effects of CO releasing molecules [tricarbonyldichlororuthenium () dimer, CORM 2] liberated CO on nitric oxide (NO) and nitric oxide syntheses (eNOS / iNOS) in monocrotaline (MCT) induced pulmonary artery hypertension (PAH ) in rats. Methods: MCT solution was given as a single subcutaneous injection (60mg/kg) to male Sprague Dawley rats. At th