Response surface method optimization of extraction process of LBP.doc

 PAGE \* MERGEFORMAT 10 Response surface method optimization of extraction process of LBP Authors: Jun-Hai, Ren Huilan, Li air damper, Li recorded [Abstract] Objective response surface method (Response Surface Methodology) of the LBP to optimize the extraction process. Method to select the single factor experiment based on the experimental factors and levels, according to Central Composite (Box-Benhnken) Principles of experimental design with three factors, the level of response surface analysis, based on regression analysis of the impact factors of various process conditions in order to extract the rate of polysaccharide response value for response surface and contour lines. The results of the analysis of the significance of each factor and interaction, results in LBP Extracting the optimum conditions are: solid-liquid ratio 1:32; extraction temperature 84 ℃ ; extraction time 2.3h; extracting a sub - . Conclusion In the optimum conditions, the actual extraction rate of LBP up to 11.10%. [Keywords:] wolfberry extract the response surface analysis of LBP Abstract: Objective To optimize the extraction technology of Lycium barbarum polysaccharides by response surface methodoloy.MethodsExperiment factors and levels were firstly selected by one factor tests. On the basis of the previous results and according to the central composite experimental design principles, the method of Response Surface Method with 3 factors and 3 levels was adopted.Then the factors influencing the technological parameters were determined by means of regression analysis.Response surface and contour were finally graphed with the extraction rate as the response value.ResultsThe optimum conditions of Lycium barbarum polysaccharides extraction were as follows: the ratio of material to solvent of 1:32, extraction temperature of 84 ℃ and extraction time of 2.3 h, one time extraction.ConclusionThe yield of Lycium barbarum polysaccharides is 11.10% under the optimum conditions. Keywords:: Lyci