Resveratrol intervention on chronic atrial fibrillation pig atria deacetylase 1 expression.docVIP

 PAGE \* MERGEFORMAT 13 Resveratrol intervention on chronic atrial fibrillation pig atria deacetylase 1 expression Of: Zhou Xiaofeng, Wang Zhirong, Zhang Zhuoqi, Maria Zhang, Xu met [Abstract] Objective resveratrol (resveratrol, RES) intervention on the rapid right atrial pacing-induced chronic atrial fibrillation (atrial fibrillation, AF) pig atria deacetylase (SIRT) 1 expression. Methods 18 small pigs (either sex) were randomly divided into 3 groups (n = 6): pacing group (ATP group) sham group (sham group) and the resveratrol treated group (RES group). using Seldinger vascular puncture puncture the right or left side of bipolar electrode into the jugular vein into the right atrium and connected to the experimental pacing (AOO), rapid atrial pacing (500 times / min) 2 weeks (sham group without pacing pigs ) Preparation of experimental model of chronic AF. RES group of pigs at 1 week before pacing start taking RES (2.5 mg kg-1 d-1). RT-PCR analysis of SIRT1 mRNA expression, Western blot detected the expression levels of SIRT1 protein . Results RES group SIRT1 mRNA and protein than the ATP group and the sham group was significantly increased (P lt;0.05), ATP set slightly higher than the sham group but the difference was not statistically significant (Pgt; 0.05). Conclusion RES intervention can significantly enhancement right atrial rapid pacing induced atrial tissue of pigs with chronic AF the expression of SIRT1. [Keywords:] antioxidants; atrial fibrillation; pig Abstract: Objective To investigate the effect of resveratrol intervention on the expression of SIRT1 in the atrial tissues in pigs with atrial fibrillation (AF) induced by right tachypacing. Methods 18 pigs of both genders were randomized into 3 groups: atrial tachypacing (ATP) group (n = 6), resveratrol-intervened (RES) group (n = 6) and sham group (n = 6). A bipolar electrode was inserted through the left or right internal jugular vein into the right atrial (RA) appendage using Seldinge