RNAi technology in cancer research and treatment applications.docVIP

 PAGE \* MERGEFORMAT 16 RNAi technology in cancer research and treatment applications [Abstract] the incidence of colon cancer in China, compared with less developed countries. But in recent years because changes in diet, the incidence tended to increase, second only to cancer tumors in the digestive tract. RNAi is a double-stranded RNA (double-stranded RNA, dsRNA)-mediated, non-coding sequence-specific degradation of corresponding mRNA and closed the small nuclease complex combination of regulation and then paired with the complementary target mRNA, preventing the corresponding mRNA, resulting in specific gene silencing. With the RNAi technology The emergence and rapid development, its application and was related to the development of colon cancer research and treatment in respect of research reports gradually increased, as in-depth study of colon cancer provides a new way of thinking. In this paper, the relevant literature review the research progress reviewed. [Keywords:] RNAi technology; colon cancer; tumor development; treatment In September 2004 lt; gt; The first time published RNA interference (RNAi)-related articles, the short period of 6 years, RNAi in biology revolutionized the field, have a great influence. Their fields in cancer research and treatment provides a more effective methods. 1 RNAi and mechanism of the molecular basis 1.1 RNAi RNAi molecular mechanism of the specific mechanism is not yet clear, but usually that RNAi involves three steps [1,2]: first, cells endogenous or exogenous long chain of double-stranded RNA (dsRNA) was named The nucleotide Dicer enzyme Ⅲ (RNase Ⅲ) is decomposed into small double-stranded RNA (including justice and anti-sense strand); the second step, a small double-stranded RNA in RNA helicase body under the action started, the formation of a single chain, which anti- guide the formation of sense strand RNA-induced silencing complex (RNA-induced silencing complex, RISC); the third step, the activati