RP-HPLC Determination of plasma drug concentration Lafutidine.docVIP

 PAGE \* MERGEFORMAT 15 RP-HPLC Determination of plasma drug concentration Lafutidine Author: Zheng, Mei Chen, Zhaochun Jing, Liu Bin Keywords: Lafutidine; blood drug concentration; RP-HPLC [Abstract] Objective: To establish Lafutidine human plasma by RP-HPLC method. Methods: Symmetry C18 column (54μm, 4.6mm × 150mm); fixed sample loop 20μL; mobile phase: methanol: 0.005mol.L diammonium phosphate = 50:50 (V: V); flow rate: 1mL . min; UV detection wavelength: 218nm; column temperature: 25 ℃. Results: lafutidine in the range of 0.506 ~ 0.01265mg.L there are a good linear relat