RP-HPLC determination of rubescens Ursolic acid and oleanolic acid content of.doc

 PAGE \* MERGEFORMAT 16 RP-HPLC determination of rubescens Ursolic acid and oleanolic acid content of 【Abstract】 Objective To establish a reversed-phase high performance liquid chromatography (RP  HPLC) determination rubescens Ursolic acid and oleanolic acid content. Method column for NUCLEODUR C18 RP column (250 mm × 4.6 mm, 5 μm); mobile phase was methanol  water (87:13); flow rate of 0.8 ml * min-1, photodiode array detector, detection wavelength 210 nm, column temperature was 25 ℃. Results Linear range of the selected chromatographic conditions were good, ursolic acid and oleanolic acid in the sample recoveries were 96.2% and 98.7%, RSD was 1.9% and 0.9%. Conclusion The method is simple, rapid, accurate and reliable. 【Key Words】 rubescens; Ursolic acid; oleanolic acid; RP-HPLC; assay Fund Project: National “863” program funded projects focus on Abstract: ObjectiveTo develop a quantitative method for determination of ursolic acid and oleanolic acid in Rabdosia rubescens by RP  HPLC. MethodsHPLC was carried out on NUCLEODUR C18 RP column (4.6 mm × 250 mm, 5 μm) at 25 ℃ using methanol  water ( 87:13) as mobile phase with a flow rate of 0.8 ml * min-1 and detection wavelength was at 210 nm. ResultsThe good linear relationship was obtained under the optimum conditions. The recoveries of the standards added for ursolic acid and oleanolic acid were 96.2% and 98.7%, respectively, while the relative standard deviations were 1.9% and 0.9%. ConclusionThe method is rapid and precise. Keywords:: Rabdosia rubescens; Ursolic acid; Oleanolic acid; RP-HPLC; Content determination Rubescens for LABIATAE Isodon Cardamine Asia Rabdosia rubescens (Hemsl.) Hara dry the whole plant is widely distributed in the Yellow River, Yangtze River, the main producing area of the Taihang Mountains in Henan Jiyuan. Rubescens sweet, bitter, slightly cold, a heat-clearing and detoxifying, anti-inflammatory pain, stomach and blood circulation and