RP-HPLC determination of tumor- capsules aloe-emodin emodin and phenolic content of rhubarb.doc

 PAGE \* MERGEFORMAT 11 RP-HPLC determination of tumor- capsules aloe-emodin emodin and phenolic content of rhubarb [Abstract] Objective To establish a tumor-Ⅰ capsules aloe-emodin, emodin and chrysophanol method for the determination. Methods using high performance liquid chromatography column was Kromasil ODS-1 (4.6 mm * 200 mm, 5μ m), using methanol -0.1% phosphoric acid (75: 25) as mobile phase, flow rate 1.0ml/min, column temperature 35 ℃ , detection wavelength 254nm. The results of aloe-emodin in the concentration range of 1.38 ~ 22.1μ g/ml, emodin in the concentration range of 1.31 ~ 21.0μ g/ml, chrysophanol in the range of 2.20 ~ 35.2μ g/ml within the peak area showed good linear relationship between correlation coefficient of 0.9999. Methods The average recoveries were 98.1% (RSD = 1.2%), 98.6% (RSD = 0.99%), and 97.4% (RSD = 1.4%). Conclusion The method is simple, accurate, reproducible, can be used as tumor Ⅰ on one of the methods of quality control capsules. [Keywords:] tumor Ⅰ Capsule; Aloe-emodin; Emodin; Chrysophanol; hplc Simultaneous determination of Aloe-emodin, Emodin and Chrysophanol in Zhongliu Ⅰ capsule by HPLC [Abstract] Objective To develop a HPLC method for simultaneous determination of Aloe-emodin, Emodin and Chrysophanol in Zhongliu Ⅰ capsule.Methods The analysis was used with a Kromasil ODS-1 (4.6mm * 200mm, 5μ m) column and a mobile phase of methanol -- 0.1% phosphoric acid solution (75:25). The flow rate was 1.0ml/min and column temperature was 35 ℃ . The detection wavelength was set at 254nm.Results The calibration curve was linear within the range of 1.38 ~ 22.1μ g/ml for Aloe-emodin, 1.31 ~ 21.0μ g/ml for emodin and 2.20 ~ 35.2μ g/ml (r = 0.9999) for Chrysophanol, the average recoveries were 98.1% (RSD = 1.2%) for Aloe-emodin, 98.6% (RSD = 0.99%) for Emodin and 97.4% (RSD = 1.4%) for Chrysophanol.Conclusion This method was convenient, accurate and reproducible for determination of Aloe-emodin, Emodin and Chrysopha