RP-HPLC method for the determination of compound Jin Pu content of the film Tetrahydropalmatine_0.doc

 PAGE \* MERGEFORMAT 9 RP-HPLC method for the determination of compound Jin Pu content of the film Tetrahydropalmatine Of: Huangming Li, Huang Dongping, Jiang your hair, Zong River [Abstract] Objective To establish a compound film of gold tetrahydropalmatine Po determination. Methods using high performance liquid chromatography column was Hypersil BDS-C18, mobile phase of methanol -5% acetic acid (40:60), and with concentrated ammonia solution adjusted to pH 4.7, flow rate 1.0 mL / min, column temperature was 25 ℃, detection wavelength 280 nm. Results Tetrahydropalmatine concentration 0.436 ~ 2.180? g range of peak area and concentration was linear, r = 0.999 7, the average recovery was 98.4%, Tetrahydropalmatine adjacent peak separation of impurities to meet the requirements. Conclusion The method is simple, specific, reproducible, and can be used for quantitative analysis of Tetrahydropalmatine. [Keywords:] compound Jin Pu tablets; Tetrahydropalmatine; high performance liquid chromatography of content; Abstract: Objective To develop a method for determination of tetrahydropalmatine in Compound Jinpu Tablets. Methods RP-HPLC method was used with Hypersil BDS-C18 column, the mobile phase was methanol-5% acetic acid (40:60) adjusted pH to 4.7 by ammonia, flow rate was 1.0 mL / min, and column temperature was 25 ℃, with the detection wavelength at 280 nm. Results The linear correlation was observed for the concentration of 0.436 ~ 2.180? g (r = 0.999 7). The average recovery was 98.4%. Resolution between tetrahydropalmatine and other peaks met the requirements. Conclusion The method is convenient, selective and reproducible for quantitative analysis of tetrahydropalmatine. Keywords:: Compound Jinpu Tablets; tetrahydropalmatine; RP-HPLC; content determination Jin Pu compound chip Cancer Institute, Guangxi Traditional Chinese medicine theory based on the development of the national drug agents, the Sweet Basil, Livistona son, Astragalus, spirulina