RPHPLC determination of biological agents Liu Wei Di Huang Tang Paeonol in.doc

 PAGE \* MERGEFORMAT 8 RPHPLC determination of biological agents Liu Wei Di Huang Tang Paeonol in [Abstract] Objective To determine the biological agents Liu Wei Di Huang Tang Paeonol content. Methods using high performance liquid chromatography: The column was UltimateTMAQ C18 column (4.6 mm 250 mm, 5 m), methanol and water (volume ratio 45:55) as mobile phase, flow rate 1 mL / min, column temperature 30 , detection wavelength 274 nm. the results of the concentration paeonol 1.024 ~ 25.60 g / mL range a good linear relationship between peak area (r = 0.999 0, n = 5), average recovery 103.3%, RSD 1.5%. Conclusion The method is accurate, specific and reproducible, and can be used for the determination of biological agents Liu Wei Di Huang Tang paeonol content. [Keywords:] Liu Wei Di Huang Tang photosynthetic bacteria paeonol Liu Wei Di Huang Tang Dynasty doctors money by the first B is a traditional Chinese nourishing kidney yin representative of the famous recipe from Rehmannia, Cornus, yam (c s) and Alisma, Dan, Poria (III diarrhea) composed of [1], the main kidney yin deficiency treatment card, since ancient times is widely used in clinical medicine [2]. The laboratory studies in the early, exploratory introduction to the photosynthetic bacteria to the classical prescription Liu Wei Di Huang Tang, the use of the nutritional content of their photosynthetic bacteria and its biotransformation to achieve greater efficacy purposes. pharmacological results show that Liu Wei Di Huang Tang Jingguang combined bacterial metabolism, the in enhancing immunity, anti-aging [3] and treatment of kidney cards such as the role model for both mice superior to the traditional Liu Wei Di Huang Tang [4]. This determination of Liu Wei Di Huang Tang establishment of biological agents in the content of the RP HPLC paeonol law, in order to further control the quality and provide experimental evidence. An instrument and reagent 1.1 Instrument Waters high performance