RT-PCR assay MYCT-1 gene in GES-1 cells.docVIP

 PAGE \* MERGEFORMAT 7 RT-PCR assay MYCT-1 gene in GES-1 cells [Abstract] Objective MYCT-1 (myc target) gene in gastric mucosa cell line GES-1 expression of GES-1 cells as a cell model of RNA interference. Methods extracted using TRIZOL GES-1 cell total RNA, RT-PCR amplification of MYCT-1 cDNA, DNA sequencing. results in GES-1 cells detected 504bp MYCT-1 cDNA fragments, sequencing confirmed with GenBank reported sequences. Conclusion GES-1 cells in the presence MYCT-1 expression, RNA interference can be used as cell model. [Keywords:] MYCT-1; GES-1 cell line; RT-PCR MYCT-1 (myc t