Saikosaponin d in alcoholic liver fibrosis of rat stellate cell activation.doc

 PAGE \* MERGEFORMAT 8 Saikosaponin d in alcoholic liver fibrosis of rat stellate cell activation Author: Su-Ting, YANG He-mei, Qi Jiemin, Meili Xin [Abstract] Objective To study the Saikosaponin  d (SS  d) of the alcohol-induced liver fibrosis in rat stellate cells (HSC) activation, to explore the SS  d anti-hepatic fibrosis in rats. Methods SD rats were randomly divided into 3 groups: alcohol group, SS-d protection group, normal control group. The use of liquor supplemented with high fat diet fed Methods Rat hepatic fibrosis model, while giving the SS  d protection, 12 rats were killed over the weekend. Immunohistochemical detection of liver tissue α  smooth muscle actin (α  SMA) expression, HE staining and Van  Gieson staining of pathological changes in liver tissue and fibrous tissue proliferation, conventional biochemical liver function were detected. Results and the model group, SS  d significantly reduced in liver tissue of α  SMA expression; reduction of serum ALT, AST activity and liver hydroxyproline (Hyp) content; significant improvement in pathological changes in liver tissue, fibrous tissue hyperplasia significantly reduced . Conclusion SS  d by reducing the expression of α  SAM, inhibit the activation of HSC to the liver on hepatic fibrosis. [Keywords:] Saikosaponin d hepatic fibrosis hepatic stellate cell α -smooth muscle actin Abstract: ObjectiveTo study the effects of saikosaponin-d (SS  d) on the activation of Hepatic Stellate Cells (HSC) and to investigate the main mechanism of SS-d in protection of liver fibrosis.MethodsSD rats were randomly divided into 3 groups: normal group, ethanol-fed group, SS  d interventional group.56% distilled alcohol and high fat diet were given orally to the rats of the ethanol-fed group and the SS-d group every day, the rats of SS  d group were injected intraperitoneally SS  d 2.0 mg * kg-1 once daily.Rats were sacrificed 12 weeks later. The expression of α  smooth muscl