Salinomycin breeding high-yielding strain of reasoning.doc

 PAGE \* MERGEFORMAT 13 Salinomycin breeding high-yielding strain of reasoning [Abstract] According to the biosynthetic pathway of salinomycin producing strain was reasoning his selection. Based on the use of conventional mutagenesis acid as a screening agent for mutants detected need to have successfully obtained three high-yielding strains of 3  233 #, 3  244 #, and 3  250 #, its effects were lower than the starting strain F2  1 # were increased by 34%, 44.9% and 39%. [Keywords:] salinomycin; mutagenic agent; screening agent; reasoning Breeding Rational screening for high salinomycin  producing strain Chu Xiao  he1, 2, Wang Pu1 and Song You  li2 (1 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032; 2 Zhejiang Shenghua Biok Biology Co., Ltd., Huzhou 313220) ABSTRACT A salinomycin  producing strain, namely # F2  1, was treated and mutated using physical factor of UV and chemical factor of ethylmethane sulfonate (EMS) respectively with reference to the inferential screening theory. The positive mutants were obtained from the plates with butyric acid for screening. Three most stable and productive strains of # 3  233, # 3  244 and # 3  250 were validated in 30 m3 fermentor. The results showed that the titres of mutant # 3  233, # 3  244 and # 3  250 were 34%, 44.9% and 39% respecti  vely, and higher than that of the original strain # F2  1. KEY WORDS Salinomycin; Mutagen; Screening reagent; Rational screening Salinomycin (salinomycin) from Streptomyces albus (Streptomyces albus) fermentation [1]. Japan has already confirmed that salinomycin had a very broad spectrum of anti-coccidiosis of the chicken tender, poisoned, and reactor type, fragile, large, and Hastelloy coccidia are valid, 50mg/kg alone significantly inhibited [2,3]. In addition, the majority of salinomycin inhibit Gram-positive bacteria, the Gram-positive anaerobes such as Clostridium also have a strong inhibitory effect [