Scanning electron microscopy of human sperm sample preparation and observation of.docVIP

 PAGE \* MERGEFORMAT 9 Scanning electron microscopy of human sperm sample preparation and observation of Authors: KONG Xiang-lin, LIU Xian Lin, Chang Aoshuang [Keywords:] sperm; microscopy, electron, scanning; ultrastructure Scanning electron microscope in clinical medicine, basic medicine and other fields a wide range of applications, particularly in leukemia, red blood cell disease, apoptosis, and other free cells of clinical diagnosis and basic research has been widely applied [1]. As the family planning and prenatal and postnatal care work, Ultrastructural study of human sperm has been wide attention. As the water content of human sperm, surface electrical conductivity is poor, therefore, scanning electron microscopy sample preparation and observation of the difficulty. In order to adapt them to the requirements of scanning electron microscope, and to better reveal its surface morphology, in the process of sample preparation need to be cleaned, dehydrated. As the surface tension of water is 72.75 dyn / cm, the sample susceptible to dehydration tension damage, shrinkage and deformation occurs [2]. At the same time, when the scanning electron microscopy, secondary electron production due to its low rate of low density samples, the electron beam easily penetrate; while samples fragile and can not stand the long electron beam irradiation. Therefore, in order to obtain better quality images, the right to adjust the scanning electron microscope work, the parameters are also very important. Through repeated pairs of infertility semen sample preparation and observation, summed up a set of better sample preparation methods, and select the appropriate accelerating voltage, to obtain better quality images. 1 Materials and methods 1.1 Instrument Vortex mixer, ordinary centrifuge, general refrigerator, JEM JEE-5B vacuum freeze-drying machine, HITCH E-1010 ion sputtering instrument, HITCHI S-3400N scanning electron microscope. 1.2 Sample Preparation 24 h period (