Salt loading in spontaneously hypertensive rat thoracic aorta collagen content and expression of TGF1.doc

 PAGE \* MERGEFORMAT 14 Salt loading in spontaneously hypertensive rat thoracic aorta collagen content and expression of TGF1 Of: Wei Na, Mu Jianjun, Wang Baoli, Liu Fuqiang, Huang Qiang, Zhang Min, Jiang Wei, Wu Guanji [Abstract] Objective Dynamic Observation of chronic salt loading in spontaneously hypertensive rats (SHR) aortic collagen content and TGF1 expression, and to explore its possible mechanism. Methods 80 SHR were randomly divided into 2 groups: 4% ( mass fraction), high-salt diet (high salt group, n = 45) and 0.4% (mass fraction) normal salt diet group (control group, n = 35), sustained intervention for 16 weeks. at intervals of 4 weeks to monitor blood pressure, urine output and urine sodium. 0,4,8,12,16 weeks of experiment were sacrificed at random SHR (each n = 6), take the thoracic aorta Masson staining, the pathological changes observed and calculated aortic collagen volume fraction (CVF), immunohistochemical determination of aortic wall of transforming growth factor 1 (TGF1) protein. Results first 4,8,12,16 week SHR aortic high-salt group than control group higher CVF [(8.02 + -1.21)% vs. (6.87 + -2.15)%, (10.35 + -2.07)% vs. (8.21 + -1.02)%, (16.20 + -1.58)% vs. (15.31 + -2.13) %, (19.37 + -1.816)% vs. (18.7 + -1.9)%, P lt;0.05]. 4,8,12 weeks of the experiment, the high salt group expression of TGF1 SHR aorta higher than the control group [( 8.02 + -1.21)% vs. (6.87 + -2.15)%, (10.35 + -2.07)% vs. (8.21 + -1.02)%, (16.20 + -1.58)% vs. (15.31 + -2.13)%, P lt;0.05]. Conclusion of chronic salt loading can SHR thoracic aorta collagen content increased, while the increase of local TGF1 levels may be the increase in collagen content caused by one of the important reasons. [Keywords:] hypertension; salt loading; collagen; transforming growth factor 1 ABSTRACT: Objective To make a dynamic observation on the effect of chronic salt loading on collagen content in thoracic aorta and the expression of TGF1 in spontaneously hypertensive rats