SDS-polyacrylamide gel electrophoresis analysis of chitosan oligosaccharide.doc

 PAGE \* MERGEFORMAT 16 SDS-polyacrylamide gel electrophoresis analysis of chitosan oligosaccharide Authors: Wang Liang, Wuchang Ying, Hua Liu, Zhang Tao 【Abstract】 Objective To study the shell of a fast and accurate analysis of the qualitative determination of the composition of oligosaccharides. Methods Continuous electrophoresis and discontinuous electrophoresis chitooligosaccharides comparative experiments to determine the best analytical conditions. The results concentrated gel concentration of 3%, separating gel concentration was 17.5%, the sample volume of 10 μl, a constant current of 40 mA, electrophoresis time of 50 min under the conditions of the best chitosan oligosaccharide analysis of patterns. Conclusion SDS  polyacrylamide gel electrophoresis analysis of the composition of chitosan oligosaccharide is indeed a simple way to quickly and efficiently qualitative detection methods. Keywords: chitosan oligosaccharide; electrophoresis analysis; polyacrylamide gel electrophoresis 【Abstract】 Objective To explore a qualitative, fast and accurate way for analyzing composition of chitooligosaccharide. Methods By consecutive electrophoresis and non  consecutive electrophoresis experiments, the best analysis conditions of chitooligosaccharide were determined. Results The concentration of plastic was 3 percent, the concentration of separation of plastic was 17.5 percent, the volume of samples was 10 μl, constant current was 40 mA, electrophoresis time was 50 min. Best analysis photos of chitooligosaccharide Can be obtained under those conditions. Conclusion The method of analyzing composition of chitooligosaccharide is truly a simple, effective, accurate and qualitative way. 【Keywords:】 chitooligosaccharide; electrophoretic analysis; polyacrylamide gel electrophoresis Shell chitosan oligosaccharide obtained by the hydrolysis product of D  glucosamine by β  (1  4) glycosidic bond connecting glucopyranosyl bond made o