Selective EGFR tyrosine kinase inhibitor gefitinib in cervical cancer cells in vitro radiosensitizing effect.docVIP

 PAGE \* MERGEFORMAT 5 Selective EGFR tyrosine kinase inhibitor gefitinib in cervical cancer cells in vitro radiosensitizing effect Author: Liu Junli, Chen Yuan, Cai Yu, ZHANG Min [Abstract] Objective To investigate the epidermal growth factor receptor tyrosine kinase inhibitor gefitinib in cervical cancer cells in vitro radiosensitizing effect. Methods Siha cervical squamous cell carcinoma cells, adenocarcinoma HeLa cells were studied using the MTT assay gefitinib right Siha and HeLa cell proliferation; the use of colony formation assay detection of gefitinib right Siha, HeLa cells, radiation sensitizing effect. Results Gefitinib can inhibit Siha, HeLa cell proliferation, the IC20 values were 0.15μ mol / L, 0.32μ mol / L, but inhibited in a dose-dependent manner; the role of gefitinib in Siha, HeLa cells, 72h after radiosensitization, respectively 1.693,1.228 than SER. Conclusion Gefitinib for cervical cancer Siha, HeLa cells have a clear radiosensitizing effect. [Keywords:] epidermal growth factor receptor; gefitinib; cervical cancer; Radiosensitization 1 Materials and methods 1.1 Drugs and reagents Gefitinib (Gefitinib, trade name IRESSA) by AstraZeneca Inc. (Macclesfield, United Kingdom) to provide, RPMI  1640 the company purchased from Gibco, fetal calf serum were purchased from Zhejiang China Sea Corporation. 1.2 Cells and culture Siha cervical squamous cell carcinoma cells, adenocarcinoma HeLa cells cryopreserved Tongji Hospital oncology laboratory. Cells under standard conditions (5% CO2, 37 ℃ ) were cultured in adherent monolayer containing 10% fetal bovine serum, 1% of the pairs of anti-culture medium RPMI  1640. 1.3 methyl thiazolyl tetrazolium (MTT) test logarithmic growth phase cells from 1.5 * 104/ml cell suspension was inoculated into 96-well plates, each hole 200μ l (ie 3 * 103 cells / hole). Placed in incubator. To be adherent cells after 2h smoke turn over a new culture medium, by adding material to continue to foster the various