常用培养基.docxVIP

常用培养基 LB培养基/升终1 X浓度胰蛋白胨10?g1.0% (w/v)酵母抽提物5?g0.5% (w/v)NaCl10?g1.0% (w/v)H2O至1升?调节pH值至7.0低盐LB培养基/升终1 X浓度 胰蛋白胨10?g1.0% (w/v)酵母抽提物5?g0.5% (w/v)NaCl10?g0.5% (w/v)H2O至1升?调节pH值至7.0Terrific Broth 培养基/升终1 X浓度胰蛋白胨12?g1.2% (w/v)酵母抽提物24?g2.4% (w/v)甘油4?g0.4% (w/v)加H2O至900 ml?灭菌，冷却至60°C后加入100 ml过滤灭菌的10X TB磷酸盐 (0.17 M KH2PO4, 0.72 M K2HPO4)SOB 培养基/升终1 X浓度胰蛋白胨20?g2.0% (w/v)酵母抽提物5?g0.5% (w/v)NaCl0.5?g0.05% (w/v)250?mM?KCl10?ml2.5?mMH2O至900 ml?调节pH值至7.0，加H2O至990 ml灭菌、冷却至室温。使用前加入10 ml灭菌的1 M MgCl2 溶液10?mMSOC 培养基SOB