常见生物模型抗氧化活性评价方法的研究进展.pdf

中国食品添加剂 ~. . . .…- 专论综主运 常见生物模型抗氧化活性评价方法的研究进展 张泽生\卢亚莉，高云峰，吴天哀，王笑仪 (天津科技大学食品工程与生物技术学院，天津 300457) 摘 要:机体的各种衰老和疾病的发生与体内自由基代谢、氧化平衡被打破有很大的关系。天然产物当中 广泛存在着高效、无毒的天然抗氧化剂，其对于维持这种平衡，减少氧化对细胞内蛋白质、脂类、核酸等生物 大分子的化学结构的破坏起到有效的防御作用。如何建立简捷、快速、有效的抗氧化模型，进而准确评价受试 物的抗氧化活性，对其在食品、医药以及日化等领域的开发应用具有显著的指导意义。本文详细归纳总结了利 用细胞、大鼠、小鼠、果蝇和秀丽隐杆线虫等生物体建立抗氧化模型方法的实验原理、建立方法以及各自生物 抗氧化模型的种类、特点特性和相关的研究进展。通过分析各类生物模型抗氧化活性评价方法的优缺点，对 抗氧化活性评价方法的发展趋势进行了分析展望。 关键词:细胞g 大鼠g 果蝇g 秀丽隐杆线虫，抗氧化活性评价 中图分类号 TS210.l 文献标识码:A 文章编号 1006-2513 (2015) 03-0178-06 Research progress on biological model of antioxidant activity evaluation ZHANG Ze-shen矿， LU Ya-li , GAO Yun-feng , WU Tian-chen , WANG Xiao-yi (Key Laboratory of Food Nutrition and Functional ingredients , Tianjin University ofScience and Technology , Minis位y of Education , Tia时in 300457) Abstract: A variety of aging and disease of human beings are connected with the unbalance of oxidation and free radical metabolism. Natural materials widely contain efficient and nontoxic antioxidants. It can reduce the strucωre damage of proteins , lipids , nucleic acids in the body due to the free radical and oxidation. Seeking for simple , rapid and effective antioxidant models to evaluate the antioxidant activity accurately has significance in guiding for the development and the application in the field of food , medicine and cosmetic etc. The cuπent research progress of cellular and other creature models of antioxidant activity assay , the experimental principles , method and their biological oxidation type and characteristic features of the model have been reviewed elaborately in this paper. The advantages and disadvantages of all kinds of antioxidant models had been analyzed. In