Several types of solid tumors of the IFNα 2a treatment of sensitivity to the relationship between the expression of cell surface receptors.docVIP

 PAGE \* MERGEFORMAT 14 Several types of solid tumors of the IFNα 2a treatment of sensitivity to the relationship between the expression of cell surface receptors Author: Liu Yan, Han Wei, Yan Zhen, ZHANG Ying-Qi [Abstract] Objective: To explore the surface of tumor cells with interferon-α receptor expression in tumor to IFN  α 2a treatment of sensitive relationships. For the establishment of interferon-α -sensitive tumors lay the foundation for the rapid evaluation method. Methods: MTT method in vitro Screening IFN  α 2a sensitive and resistant tumor cell lines, select the human lung adenocarcinoma cells A549, SPC  A  1, GLC  82 and human poorly differentiated gastric cancer cell MKN  45 a total of four cells; by constructing tumor-bearing nude mouse model, confirmed that different types of tumor sensitivity to interferon treatment are different. flow cytometry, immunohistochemistry, Western Blot detection of tumor cell surface expression of IFN  α receptor differences. Results: in vivo, in vitro results showed that: A549 , SPC  A  1 for IFN  α 2a-sensitive cell lines, MKN  45, GLC  82 for IFN  α 2a resistant cell line. cell and tissue levels of test results showed that: the expression of high to low order of A549, SPC  A  1, MKN  45, GLC  82. Conclusion: IFN  α receptor expression correlated with the sensitivity of tumor cells to interferon is closely related to the achievement of targeted individual treatment of have some significance. [Keywords:] Interferon-α ; receptors, interferon-α ; sensitivity and specificity of [Abstract] AIM: To explore the relationship between interferon  α receptor expression on tumor cell surface and tumor sensitivity to IFN  α 2a treatment so as to develop a new method for quick identification of IFN  α sensitive tumors. METHODS: MTT assay was adopted to differentiate the sensitive and resistant cell lines to IFN  α . We chose four cell lines, human lung adenocarcinoma (