Sinomenine on adjuvant arthritis inflammatory and immune function in rats with.docVIP

 PAGE \* MERGEFORMAT 13 Sinomenine on adjuvant arthritis inflammatory and immune function in rats with Author: Chen Fang-jun Ye Cheng Yuan-Hua Hu Wei, Zhang Rongyi [Abstract] [Objective] sinomenine (SINO) on adjuvant arthritis (AA) rats with inflammatory and immune function. [Method] selected SD rats were randomly divided into normal group, model group, SINO low, middle and high dose group (dose, respectively 60,120,240 mg / kg), Tripterygium glycosides (TPT) group (dose of 30mg / kg); In addition to the normal group, other groups are used Freund’s complete adjuvant, paw intradermal injection of replication AA model; modeling of each group in the starting 12 days after delivery, continuous 12d; the effects of different time periods of the rat paw toe swelling, and joint score; using radioimmunoassay synovial cell interleukin  1β (IL  1β ), tumor necrosis factor  α (TNF  α ) of the content; to observe the synovial cells in rats AMD structure; using reverse transcription - polymerase chain reaction detection of synovial cells in each group IL  1β mRNA, TNF  α mRNA expression. [Results] SINO-dose group can be reduced to varying degrees in rats toe swelling and arthritis score inhibit synovial cells to produce IL  1β , TNF  α , reduced synovial cells IL  1β mRNA, TNF  α mRNA expression of (P amp;lt;0.05 or P amp;lt;0.01), to restore AA rat synovial cell morphology. [Conclusion] SINO role in the treatment of rheumatoid arthritis synovial cells may be related to able to inhibit secretion of inflammatory mediators and restore the synovial cell morphology. [Keywords:] sinomenine / pharmacology; arthritis, rheumatoid / TCD therapy; arthritis, rheumatoid / immunology; synovial cells / ultrastructure; Disease models, animal; rat Rheumatoid arthritis (rheumatoid arthritis, RA) is a chronic systemic autoimmune disease, the pathological basis of joint synovitis, the cause is unknown, complex and diverse clinical manifestations, serious harm to hu