Spleen Spleen Qi Prescription on asthmatic rats IκB - NF-κB signaling pathway in.docVIP

 PAGE \* MERGEFORMAT 13 Spleen Spleen Qi Prescription on asthmatic rats IκB / NF-κB signaling pathway in [Abstract] Objective To investigate the spleen induced airway inflammation in asthma, blood and bronchoalveolar lavage fluid (BALF), the percentage of eosinophils in lung tissue nuclear factor-κB inhibitor protein @ (IκB @) expression and nuclear factor-κB (NF-κB) activity in spleen and replenishing qi and its effects. Methods 30 male Wistar rats were randomly divided into 3 groups: normal control group (A group), spleen asthma group (B group), spleen and replenishing qi group (C group), HE staining of lung tissue with pathological changes detected by immunohistochemistry in lung tissue IκB @ expression and activity of NF-κB p65. Results A group B group, lung Organization IκB @ was significantly reduced, while NF-κB p65 activity was significantly higher (P lt;0.01), C group and B group, lung tissue IκB @ expression was significantly increased, while NF-κB p65 activity was significantly decreased (P lt;0.01 ). Conclusion Spleen Qi deficiency prescription asthma can effectively increase IκB @ lung tissue expression, inhibition of NF-κBp65 lung tissue activity, to reduce airway inflammation in asthma changes, and thus play a spleen asthma treatment effect. [Keywords:] deficiency, asthma, nuclear factor-κB inhibitory protein @, nuclear factor-κB, rat Abstract: Objective To investigate airway inflammation, number of eosinophils in blood and BALF, the expression of inhibitor protein @ (I κB @) and nuclear factor-κB (NF-κB) activity in lung of rat with spleen deficiency asthma, and the effect of herbs of reinforcing spleen to replenishing Qi. Method Thirty rats were randomly divided into three groups: control group (group A), spleen deficiency asthma group (group B), decoction group (group C). The pathological changes of lung was detected by HE staining, and the expression of I κB @ and activity of nuclear factor κB p65 (NF-κB p65) was assessed in lung by