The external source of radiation injury of bone marrow-derived mesenchymal stem cells the impact of migration Colonization.docVIP

 PAGE \* MERGEFORMAT 24 The external source of radiation injury of bone marrow-derived mesenchymal stem cells the impact of migration Colonization 【Abstract】 The central nervous system by radiation injury of bone marrow-derived mesenchymal stem cells (MSCs) Colonization characteristics of changes in migration, so as to application of the treatment of central nervous system radiation injury of MSCs to provide theoretical and experimental basis. 【Methods】 The 8-week-old SD rats received 22GyX-ray radiation produced central nervous system radiation injury model; from GFP mouse bone marrow, separation and amplification of MSCs. The central nervous system radiation injury in SD rats by 4 months after I intravenous infusion of GFP-MSCs. At several time points after transplantation to take the brain, bone marrow, liver, spleen, etc. to do frozen section, using two-photon infrared pulsed laser confocal microscopy GFP-MSCs. 【Results】 In the first 24 h within the experimental group and control group was detected in the bone marrow of exogenous GFP-positive cells to 7 days after the experimental group, GFP-positive cells in the bone marrow of significantly more than the control group. Day 1 brain does not detect exogenous GFP-positive cells, 4 days after radiation injury in the central nervous system by the SD rats, brain tissue with a green fluorescence to detect exogenous GFP-positive cells; while the control group, the brain has not been detected with a green fluorescent exogenous MSCs. The experimental group and control group in the spleen 1 day after infusion of MSCs that is detected by a large number of GFP-positive cells, and the persistence of the first 21 days of the experimental group than in the control group of GFP-positive cells decrease. 【Conclusion】 radiation injury under the conditions of exogenous MSCs through the blood-brain barrier to reach brain tissue, central nervous system radiation injury can be induced by exogenous MSCs migrate to the brain