Vero cells inactivated hantavirus vaccine virus seed candidates for screening.docVIP

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2017-05-05 发布于浙江
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Vero cells inactivated hantavirus vaccine virus seed candidates for screening.doc

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Vero cells inactivated hantavirus vaccine virus seed candidates for screening

Vero cells inactivated hantavirus vaccine virus seed candidates for screening [Abstract] purposes hemorrhagic fever with renal syndrome vaccine strain of back-up filter adapted to Vero cells, and its antigenicity and immunogenicity study. Methods isolated four new hantavirus vaccine-sensitive animals, in the neonatal gerbil brain continuous passage, compare the content of the brain, as well as viral antigens incidence of newborn mice; passaged in Vero cells, comparing the culture medium of virus drops degree and antigen titers, as well as cell lesions; the use of selected strains of Vero cells, inactivated vaccine developed to study the immunogenicity of the vaccine. Results 4 virus by neonatal gerbil brain passaged mouse brain suspension of virus titer and antigen titers respectively 7  00 ~ 7  75LgCCID50/ml and 1:320 ~ 1:1280; 4 strain by Vero cells pass five consecutive generations, culture fluid virus titer and antigen titers of respectively 6  50 ~ 7  50LgCID50/ml and 1:160 ~ 1:768; two needles in rabbits after vaccination, in which strains of hemorrhagic fever with ZJ4 ZJ5 strains of vaccine-type strains of the same serum neutralization titer to 1:10. Conclusion ZJ4 and ZJ5 two strains have been adapted to Vero cells, and has a high titer virus and immunogenicity of the good characteristics, suitable for use as Vero cells, hemorrhagic fever with renal syndrome vaccine strain an ideal candidate. [Keywords:] Hantavirus Hemorrhagic fever with renal syndrome (HFRS) is a serious hazard to human health of natural foci of disease. HFRS in a variety of preventive measures, the vaccine is one of the most specific and most effective means of prevention. To date, this laboratory model has been developed HFRS Ⅰ , Ⅱ -type and double-price and purified bivalent inactivated vaccine gerbil kidney and has been put into mass production. The field epidemiology investigations, the protection of people affected areas the rate of 95%, and for the prevention of HFRS has