儿茶素对5-6肾切除大鼠肾脏微血管的保护作用及其机制.docVIP

　　儿茶素对5/6肾切除大鼠肾脏微血管的保护作用及其机制 作者：曹艳, 何小解, 向伟, 易著文 【摘要】 目的：探讨儿茶素对5/6肾切除大鼠血管紧张素转化酶（angiotensinconverting enzyme, ACE）、血管紧张素Ⅱ（angiotensin Ⅱ, Ang Ⅱ）及肾脏组织微血管密度（microvessel density, MVD）的影响。方法：60只SD大鼠被分为假手术组、模型组和儿茶素组。模型组和儿茶素组大鼠切除5/6肾脏制作模型，假手术组不做肾切除。儿茶素 治疗 8周与12周后，应用免疫组织化学法检测CD34标记的肾局部微血管数并 计算 密度；紫外分光光度法检测血浆与肾皮质中ACE活性；放射免疫法测定血浆与肾皮质中Ang Ⅱ浓度；苏木精和伊红（hematoxylin and eosin，HE）染色及过碘酸希夫（periodic acidSchiff, PAS）染色后，半定量积分法评价肾小球硬化指数(glomerular sclerosis index, GSI)与肾小管间质损伤指数(tubule interstitial score, TIS)。结果：儿茶素组GSI与TIS在第8、12周末均显著低于模型组（Plt;0.05，Plt;0.01）；模型组和儿茶素组肾小球及小管周围MVD在第8周与第12周末较假手术组均明显降低（Plt;0.01）；儿茶素组血浆、肾皮质中ACE活性与Ang Ⅱ浓度在实验第8与第12周末均显著低于模型组（Plt;0.01）；Pearson相关分析发现，MVD与GSI、TIS、ACE、Ang Ⅱ呈负相关（Plt;0.01），ACE、Ang Ⅱ与GSI、TIS呈正相关（Plt;0.01）。 结论：儿茶素可有效阻止5/6肾切除大鼠肾脏微血管密度的减少，抑制肾小球硬化与肾间质纤维化的进展，这可能与儿茶素抑制ACE的活性，减少Ang Ⅱ的生成有关。 【关键词】 儿茶素; 微血管密度; 血管紧张素Ⅱ; 血管紧张素转化酶; 大鼠 Objective: To investigate the effects of catechin on angiotensinconverting enzyme (ACE) activity, angiotensin Ⅱ(Ang Ⅱ) content and microvessel density (MVD) in renal tissues of 5/6 nephrectomized rats. Methods: Sixty SD rats operated group, untreated group and catechin group. Animal model y. After 8 and 12inistration, rats easured by immunohistochemical method arking. Activities of ACE in plasma and renal cortices easured by ultraviolet spectrophotometer, and Ang Ⅱ contents in plasma and renal cortices easured by radioimmunoassay. Glomerular sclerosis index (GSI) and tubule interstitial score (TIS) iquantitative integration atoxylin and eosin staining and periodic acidSchiff staining. Results: After 8 and 12inistration, the GSI and TIS in the catechin group uch less than those in the untreated group (Plt;0.05, Plt;0.01). MVDs around glomerulus and tubule at the end of the 8th and 12th uch less than those in the shamoperated group (Plt;0.01). The ACE activities and Ang Ⅱcontents in plasma and renal cortices in the catechin group uch less than those in the untreated group (Plt;0.01). By Pearson correlation analysi