血管紧张素Ⅱ对脐血CD34+细胞体外分化为巨核细胞的影响.docVIP

　　血管紧张素Ⅱ对脐血CD34+细胞体外分化为巨核细胞的影响 作者：戴兵，何吉，陈舒，刘晋辉，秦斐，朱发明，严力行 【摘要】 　　为了探讨血管紧张素Ⅱ对脐血CD34+细胞诱导分化为巨核细胞的 影响 ，采用免疫磁珠法(MACS)分选8例健康产妇足月顺产胎儿脐血中的CD34+细胞，在含血小板生成素（TPO 50 ng/ml）、白介素-3（IL-3 10 ng/ml）、干细胞刺激因子（SCF 50 ng/ml）的无血清培养液中添加浓度分别为50、100、1 000 μg/ml的血管紧张素Ⅱ作为实验组；同时以未添加血管紧张素Ⅱ的基础培养液作为对照组，培养14天后观察结果。细胞计数仪计数单个核细胞数（MNC）；流式细胞仪计数培养体系中的CD41+细胞数、血小板数，及 分析 细胞周期；利用CD41单克隆抗体免疫荧光染色观察培养体系中的细胞情况。结果表明： 与对照组比较，实验组单个核细胞数无明显改变（Pgt;0.05）；而CD41+细胞和血小板数量有明显的增加(Plt;0.05)；细胞周期分析显示，实验组的4倍体细胞增加，并存在明显的凋亡(Plt;0.05)；荧光显微镜下观察对照组和实验组均可见大小不一的CD41+细胞。结论： 血管紧张素Ⅱ可以促进脐血中CD34+细胞诱导分化为巨核细胞，并能促进巨核细胞产生血小板。 【关键词】 血管紧张素Ⅱ；CD34+细胞；巨核细胞；脐血 　　Effect of Angiotensin Ⅱ on Differentiation of Umbilical Cord Blood CD34+ Cells into Megakaryocytes 　　Abstract This study ed to investigate the effect of angiotensin Ⅱon differentiation of cord blood CD34+ cells into megakaryocytes in vitro. The CD34+ cells from Eight fresh umbilical cord blood samples sorted by a high-gradient magic cell sorting system (MACS) -free culture medium containing thrombopoietin (TPO) 50 ng/ml，IL-3 10 ng/ml，stem cell factor (SCF) 50 ng/ml and different concentrations of angiotensinⅡ(0，50，100，1000 μg/ml) for 14 days. Mononuclear cells (MNC) atic cell analyzer. Cultured CD41+ cell and platelet counts in cultured system，and cell cycle etry. CD41 specific monoclonal antibody staining munofluorescence microscopy. The results shober of MNC not increased significantly（Pgt;0.05），but the number of CD41+ cells and platelets increased significantly in treatment group (Plt;0.05). Cell cycle analysis revealed that the amounts of 4N cells increased and apoptosis cells obviously existed in treatment group (Plt;0.05). After fluorescence staining，more CD41+ cells of different sizes eans of fluorescence microscopy in both groups. It is concluded that angiotensinⅡ can induce the cord blood CD34+ cells to differentiate toegakaryocyte，and enhance the function of megakaryocyte to produce platelet. 　　Key egakaryocyte； umbilical cord blood 　　血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）是肾素血管紧张素系统的一种