Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b from paid blood donors experienced anti-retroviral therapy in chinese central province.docVIP

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Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b from paid blood donors experienced anti-retroviral therapy in chinese central province.doc

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Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b from paid blood donors experienced anti-retroviral therapy in chinese central province

Quetal.VirologyJournal2010,7:280 /content/7/1/280 RESEARCH OpenAccess Co-receptorusageandpredictionofv3 genotypingalgorithmsinhiv-1subtypeb’from paidblooddonorsexperiencedanti-retroviral therapyinchinesecentralprovince * ShuilingQu,LiyingMa,LinYuan,WesiXu,KunxueHong,HuiXing,YangHuang,XiaolingYu,YimingShao* Abstract Background:Thisstudyexploredco-receptorusageandpredictionofV3genotypingalgorithmsinHIV-1subtype B’frompaidblooddonorsexperiencedanti-retroviraltherapyinChinesecentralprovinceinordertodesign effectivelytherapeuticregimen. Methods:HIV-1strainswereisolatedintreatmentHIV-1infectionsandtreatment-na?veHIV-1infections,thenco- receptorusageofHIV-1strainswasidentifiedbasedonGhostcelllinesusingflowcytometry.HIV-1V3regionwas amplifiedandsubmittedintoweb-server(WebPSSMandgeno2pheno)topredictHIV-1co-receptorusage.The feasibilityofpredictionHIV-1usagewithWeb-serverassaywasanalyzedbycomparingpredictionofV3genotyping algorithmswithHIVphenotypeassaybasedonGhostcellline. Results:45HIV-1strainsand114HIV-1strainswereisolatedfromHIV-1infectionsexposedanti-retroviraltherapy andtreatment-na?ve,respectively.41%clinicalvirusesfromARTpatientsand18%fromtreatment-na?vepatients usedCXCR4asco-receptor.ThenetchargeintheV3loopwassignificantlydifferenceinbothgroups.The sensitivityandspecificityforpredictingco-receptorcapacityis54.6%and90.0%on11/25rule,50.0%and90%on Web-PSSMx4r5,68.2%and40.0%onGeno2pheno[co-receptor] . Conclusion:Dual/mixed/X4co-receptorutilizationwashigherinARTpatientsthantreatment-na?vepatients.Itis shouldpaidattentiontopredictingHIV-1co-receptorusagebasedonV3genotypingalgorithmsinHIV-1subtype B’frompaidblooddonorsexperiencedanti-retroviraltherapyinChinesecentralprovince. Background swarm[2].Therearestill50%patientswithlatestageHIV- HIV-1entersahostcellusingtheCD4receptorandco- 1BinfectionhavingonlyR5virusesdetectableintreat- receptorsincludingtheCXCR4and/orCCR5.Ingeneral, ment-na?ve HIV-1 patients[3] but not other HIV-1 R5-tropicstrainsusingCCR5asco-recept