Comparison of Chloroplast Genomes of English Yew (Taxus baccata L.) and Japanese Black Pine (Pinus thunbergii Parl.).docVIP

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Comparison of Chloroplast Genomes of English Yew (Taxus baccata L.) and Japanese Black Pine (Pinus thunbergii Parl.).doc

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Comparison of Chloroplast Genomes of English Yew (Taxus baccata L.) and Japanese Black Pine (Pinus thunbergii Parl.)

Acta Silv. Lign. Hung., Vol. 8 (2012) 159–164 Comparison of Chloroplast Genomes of English Yew (Taxus baccata L.) and Japanese Black Pine (Pinus thunbergii Parl.) a? Norbert FRANK – Berthold HEINZEb a Institute of Silviculture and Forest Protection, Faculty of Forestry, University of West Hungary, Sopron, Hungary Department of Genetics, Federal Research Centre for Forests, Natural Hazards and Landscape (BFW), Vienna, Austria b Abstract – Previous studies based on of allozyme polymorphisms in Taxus baccata (English yew) have been concerned mainly with inheritance analysis and genetic differentiation within and among yew stands. Our primary goal was to find chloroplast primer restriction enzyme combinations which reveal genetic polymorphisms in the chloroplast genome of samples from Central Europe but no polymorphisms were detected. However, we were able to map location of successful primer combinations on the published chloroplast genome of Japanese black pine (Pinus thunbergii) and to compare the primer positions with those of two other completely sequenced conifer genomes. Yew sequences in GenBank were also placed onto our map. As we were able to amplify a number of hitherto unexplored spacers between genes, the map of chloroplast structure shows that gene order is likely to be identical between Taxus and Pinus over large parts of the chloroplast. similarity / chloroplast / English yew / genetic polymorphism Kivonat – Az európai tiszafa és a japán feketefeny? cpDNS ?sszehasonlító vizsgálata. Korábbi tanulmányok az európai tiszafa ( Taxus baccata) izoenzim polimorfizmus ?r?kl?désének elemzésével, az állományokon belüli és állományok k?z?tti genetikai differenciálódásával foglalkoztak. Vizsgálataink els?dleges célja olyan kloroplasztisz restrikciós enzimek azonosítása, amelyekkel a k?zép-európai minták genomjának polimorfizmusa detektálható.