Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands.docVIP

Virology Journal BioMedCentral Short report Open Access Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands Van-Mai Cao-Lormeau Address: Head, Laboratoire de Recherche en Virologie Médicale, Institut Louis Malardé, Po Box 30, 98 713 Papeete, Tahiti, French Polynesia Email: Van-Mai Cao-Lormeau - mlormeau@ilm.pf Published: 25 March 2009 Received: 8 October 2008 Accepted: 25 March 2009 Virology Journal 2009, 6:35 doi:10.1186/1743-422X-6-35 This article is available from: /content/6/1/35 ? 2009 Cao-Lormeau; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Dengue virus (DENV), the etiological agent of dengue fever, is transmitted to the human host during blood uptake by an infective mosquito. Infection of vector salivary glands and further injection of infectious saliva into the human host are key events of the DENV transmission cycle. However, the molecular mechanisms of DENV entry into the mosquito salivary glands have not been clearly identified. Otherwise, although it was demonstrated for other vector-transmitted pathogens that insect salivary components may interact with host immune agents and impact the establishment of infection, the role of mosquito saliva on DENV infection in human has been only poorly documented. To identify salivary gland molecules which might interact with DENV at these key steps of transmission cycle, we investigated the presence of proteins able to bind DENV in salivary gland extracts (SGE) from two mosquito species. Using virus overlay protein binding assay, we detected several proteins able to bind DENV in SGE from Aedes aegypti (L.) and Aedes polynesiensis (Marks). The present findings pav