Development and characterization of polymeric nanoparticles as Barbatim_o (Stryphnodendron obovatum) standardized fraction carrier.docVIP

Advances in Bioscience and Biotechnology, 2013, 4, 89-92 ABB /10.4236/abb.2013.41013 Published Online January 2013 (/journal/abb/) Development and characterization of polymeric nanoparticles as Barbatim?o (Stryphnodendron obovatum) standardized fraction carrier Daniel F. G. Costa, Ju?ara R. Franca, Tatiana G. Ribeiro, Maria Auxiliadora C. Kaplan, André A. G. Faraco, Rachel O. Castilho * Department of Pharmaceutical Products, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil * Email: roc2006@farmacia.ufmg.br Received 19 November 2012; revised 25 December 2012; accepted 6 January 2013 ABSTRACT of ROS, either by a primary event (excessive exposure to radiation) or a secondary one (tissue damage by trauma) [3]. Among these diseases are those which affect the skin such as psoriasis, dermatitis and burns. Recent studies have shown that Barbatim?o fractions are promising for dermatological diseases treatment, due to tannins content and its strong antioxidant activity. In this study, chitosan was used as polymeric matrix for the development of nanoparticulated sys- tem able to deliver a Barbatim?o standardized frac- tion. Nanoparticles were prepared by ionotropic ge- lation method involving the mixing of two aqueous phases at room temperature. The product was char- acterized by infrared absorption spectrometry, scan- ning electron microscopy, quantification of fraction in the formulation, in vitro release profile, size of parti- cles and polydispersity index. The level of Barba- tim?o fraction obtained in the formulation was 38.23%, with 30% of encapsulated fraction released in 7 days. No chemical reaction between the fraction and the matrix could be noticed. The particles con- taining fraction presented an average size of 241 nm and polydispersity index of 0.484. It seems that the phytotherapic formulation could be used as drug de- livery system for sustained release