Effect of Gamma Irradiation on Regeneration Capacity of Mangosteen Nodular Callus.docVIP

HAYATI Journal of Biosciences, December 2007, p 140-144 ISSN: 1978-3019 Vol. 14, No. 4 Pengaruh Iradiasi Sinar Gamma terhadap Kapasitas Regenerasi Kalus NodularTanaman Manggis Effect of Gamma Irradiation on Regeneration Capacity of Mangosteen Nodular Callus , GULDOF ALBERT WATTIMENA , WITJAKSONO WARID ALI QOSIM1?, ROEDHY PURWANTO2 2 3 1 Departement of Agronomy and Plant Breeding, Faculty of Agriculture, University of Padjadjaran Jalan Raya Jatinangor Km. 21, Ujung Berung, Bandung 40600, Indonesia Departement of Agronomy, Faculty of Agriculture, Bogor Agriculture Univesity, Darmaga Campus, Bogor 16680, Indonesia 2 3 Botany Division, Research Center for Biology, The Indonesian Institute of Science, Jalan Ir. H. Juanda 22, Bogor 16122, Indonesia Received January 12, 2007/Accepted November 12, 2007 The research was conducted to determine the effect of gamma irradiation on regeneration capacity of mangosteed nodular callus. Nodular calli derived from a leaf as explants and cultured on MS medium containing combination of 2.2 μM benzilaminopurin (BAP) and 2.27 μM tidiazuron (TDZ). Nodular calli were irradiated with 0 (control) 5, 10, 15, 20, 25, 30, 35, and 40 Gy doses of gamma irradiation. After the irradiation, the calli were generated on woody plant medium (WPM), supplemented with 1.39 μM polyvinilpirolidon (PVP), 8 g.l agar, 30 g.l sucrose and 2.2 μM BAP concentration. -1 -1 Results showed that the irradiation influence the plant regeneration. Response dose of 50% (RD) that could promote the nodular calli of shoot formation was the 25 Gy while that of the shoot number per nodular calli was the 21 Gy. The shoot number irradiated with total dose 5 Gy (9.1 shoot) was higher than that of 0 Gy (8.6 shoot). Key words: pla