不同强度静磁场对成骨细胞细胞形态和细胞表面超微-第三军医大学学报.doc

静磁场对成骨细胞增殖活性和细胞表面超微结构的影响 王胜国 1周力2王艳民2 陈扬熙2 任晓华3重庆医科大学附属第二医院重庆400010；2 四川大学华西口腔医学院口腔正畸科 成都610041四川省人民医院口腔科 成都 610072 [摘要]目的 观察不同强度和作用时间的静磁场对体外培养成骨细胞的增殖活性及其表面超微结构的影响，探索静磁场加载对成骨细胞增殖等功能活性影响的机制。方法 将体外培养成骨细胞分别于静磁场加载装置0、8、50、160mT磁场强度下给予24、48、72h的静磁场加载。应用MTT法检测成骨细胞的增殖活性；应用倒置相差显微镜和扫描电镜观察细胞形态及其表面超微结构的变化。结果 与不给予静磁场加载对照组比较，培养成骨细胞分别于8、50、160mT静磁场加载24、48、72h后，其增殖活性出现不同程度的增加；其细胞形态、数量及分布上无差异；而随加载时间延长，细胞表面变得凹凸不平，微绒毛增多增粗增大，可见融合成囊泡及大囊泡，且8mT和50mT静磁场的作用明显。结论 8、50、160mT静磁场随加载时间延长，体外培养成骨细胞增殖活性出现不同程度增加细胞表面超微结构改建。 [关键词]静磁场；成骨细胞；增殖活性；超微结构；扫描电镜 [中图分类号] R783.5 [文献标志码] A The effect on the proliferation and cell surface ultra structure of the osteoblast during static magnetic field loading Wang Shengguo1, Zhou Li2 , Chen Yangxi2, Wang Yanmin2 , Ren Xiaohua 3 (1Department of stomatology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010; 2Department of orthodontics, WestChina college of stomatology, Sichuan University, Chengdu,610041; 3 Department of stomatology ,The people’s hospital of Sichuan province, Chengdu, 610072, China) [Abstract] Objective To observe the proliferation and cell surface ultra structure change of osteoblast during static magnetic field loading. Methods The osteoblast was cultured under 0mT, 8mT，50mT and 160mT static magnetic field for 24，48 and 72 hours; the 0mT was used as control. The MTT method was used to detect the proliferation；Cell shape and cell surface ultra structure of osteoblast were observed under inverted phase contrast microscope and Scanning electric one. Result After cultured under static magnetic field, the proliferation activity increased differently according to the magnitude, No change was found in osteoblast shape, magnitude and distribution during static magnetic field loading under inverted phase contrast microscope; but the osteoblast surface ultra structure rebuilding were observed during static magnetic field loading under scanning electric microscope, the static magnetic field loading osteoblast show