Antibiotic Resistance of Bacteria Isolated from the Internal Organs of Edible Snow Crabs.docVIP

Antibiotic Resistance of Bacteria Isolated from the Internal Organs of Edible Snow Crabs.doc

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Antibiotic Resistance of Bacteria Isolated from the Internal Organs of Edible Snow Crabs

AntibioticResistanceofBacteriaIsolatedfromthe InternalOrgansofEdibleSnowCrabs MisoonKim1,Tae-HyungKwon1,Su-MiJung2,Seung-HakCho2,SeonYeongJin1,Nyun-HoPark1, Choong-GonKim1,Jong-ShikKim1* 1GyeongbukInstituteforMarineBioindustry,Jukbyeon-Meon,Uljin-Gun,Gyeongbuk,RepublicofKorea,2DivisionofEntericBacterialInfections,CenterforInfectious Diseases,KoreaNationalInstituteofHealth,Cheongwon-Gun,Chungcheongbuk,RepublicofKorea Abstract Antibiotic resistance and microbiota within edible snow crabs are important for the Chionoecetes (snow crab) fishing industry.Weinvestigatedtheseparametersusingculturemethodsandantibioticsusceptibilitytestswithsixinternalorgans fromthreespeciesofChionoecetes.EachsamplerevealedmanyunexpectedmicrobialspecieswithinChionoecetesinternal organs.Onthebasisof16SrRNAsequenceanalysisof381isolates,themostabundantgeneraidentifiedinChionoecetes opiliowereAcinetobacterspp.(24%),Bacillusspp.(4%),Pseudomonasspp.(34%),Stenotrophomonasspp.(28%),andAgreia spp.(11%).InChionoecetessp.crabs,Acinetobacterspp.(23%),Bacillusspp.(12%),andPsychrobacterspp.(20%)weremost prevalent,whileAgreiaspp.(11%),Bacillusspp.(31%),Microbacteriumspp.(10%),Rhodococcusspp.(12%),andAgrococcus spp.(6%)weremostabundantinC.japonicus.Ourantibioticresistancetestfoundresistancetoallnineantibioticstestedin 19,14,andtwooftheisolatesfromC.opilio,Chionoecetessp.,and,C.japonicusrespectively.Ourresultsarethefirsttoshow thatmicrobeswithantibioticresistancearewidelydistributedthroughouttheinternalorgansofnaturalsnowcrabs. Citation:KimM,KwonT-H,JungS-M,ChoS-H,JinSY,etal.(2013)AntibioticResistanceofBacteriaIsolatedfromtheInternalOrgansofEdibleSnowCrabs.PLoS ONE8(8):e70887.doi:10.1371/journal.pone.0070887 Editor:DongshengZhou,BeijingInstituteofMicrobiologyandEpidemiology,China ReceivedJanuary29,2013;AcceptedJune25,2013;PublishedAugust21,2013 Copyright:?2013Kimetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproduc

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